Table 4. Final results obtained on the combined data set (Sanger + MiSeq), after completion of the workflow.
| Gene region | COI | Cytb | EF | |
|---|---|---|---|---|
| Sanger | Number of specimens with a valid sequence | 195 | 264 | 203 |
| MiSeq | Number (%) of specimens/species with at least one consensus sequence | 306 (82.9%) [incl. 27 with PCR−]/109 (94.8%) | 261 (70.7%) [incl. 13 with PCR−]/95 (82.6%) | 273 (74.0%) [incl. 45 with PCR−]/97 (84.3%) |
| Number of specimens/species with PCR+ but no sequence | 141/22 | 18/4 | 9/3 | |
| Number of specimens/species with PCR− but at least one consensus sequence | 273/94 | 14/3 | 45/11 | |
| Number of specimens with two valid sequences | 1 (0.3%) | 8 (3.1%) | 0 | |
| Number of problematic species (See text for discussion) | 3 (2.6%) | 4 (3.5%) | 0 | |
| 2 (1.7%) | ||||
1At least one of the two PCR reactions (COI-short or COI-long) was positive.
2At least one of the two PCR reactions was positive for at least one specimen.
3The two PCR reactions were negative.
4The two PCR reactions were negative for all specimens.