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. 2017 Feb 6;7:41939. doi: 10.1038/srep41939

Figure 1. ER stress markers in cGVHD target organs.

Figure 1

(A) qPCR analysis of GRP78 in cGVHD-affected organs (red) and control subjects (blue). Data from one of two similar experiments are shown. The data are presented as means, ±SD, Control: n = 5, cGVHD: n = 4–5 *P < 0.05. (B) Immunoblot analysis of ER stress indicators and the associated inflammatory molecules was carried out. (Lanes 1, 3, 5, 7: Syngeneic control subjects, Lanes 2, 4, 6, 8: cGVHD-impaired organs) Cropped blots are displayed. (C) The corresponding quantitative analysis of the protein bands was conducted. cGVHD-affected organs (red) and control subjects (blue). Data from one of two similar experiments are shown. The data are presented as means, ±SD, Control: n = 4, cGVHD: n = 4 *P < 0.05. (D) Electron micrographs of epithelial cells in the cGVHD-affected lacrimal gland and those in its syngeneic control counterpart. The pictures were taken at 2000x magnification, and the scale bar is 5 μm. In the image of cGVHD-affected lacrimal gland epithelia, asterisks are placed where the ER was expanded due to the accumulation of proteins.