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. 2017 Feb 6;7:42025. doi: 10.1038/srep42025

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(a) Representative example of intact, untreated, permeabilized and fixed cells immunostained for ABCG1-myc (green) and the ER marker calreticulin (red). (b) Green (I_G) and (c) red (I_R) channels images and (d) the difference (I_G-I_R) image. (e) The variances of the ACF of the difference images (n = 12) are larger than that of green channel alone, indicating that ABCG1 that did not co-localize with ER tended to be filamentous in nature. (f) 2D Kurtosis displays lower peakedness in the difference image than in the green channel alone ^**P < 0.01.