Fig. 3.

Induction of mitophagy in young and senescent fibroblasts by CCCP. Twenty-four hours after treatment with the chemical uncoupling agent CCCP, PINK1 levels in young and senescent fibroblasts were assessed by immunoblotting (A). PINK1 levels in untreated controls (Con) were set to 100% (dark grey bars). PINK1 amount after CCCP treatment is shown in relation to the untreated control. DMSO serves as solvent control for CCCP. The insert shows the PINK1 levels in untreated young and senescent controls. Panel B demonstrates the uptake of mitochondria by lysosomes (mitophagy flux) after CCCP treatment in young and senescent cells. Statistical significance differences are indicated by ‘*’.