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. 2017 Jan 25;11:673–681. doi: 10.1016/j.redox.2017.01.017

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© 2017 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 7. — Effects of Lon protease downregulation on lipofuscin formation during SIPS. HeLa cells were stably transfected with a doxycycline (Dox) inducible vector expressing a short hairpin RNA against Lon protease. Successful Lon protease downregulation was verified by immunoblot (A). Panel B represents lipofuscin accumulation after PQ-induced SIPS in Lon-deficient HeLa cells. Autofluorescence intensities were measured by Cell Lab Quanta™ SC MPL flow cytometer (Beckman Coulter, Krefeld, Germany) (Ex: 360 nm, Em: 252 nm). Panel C shows lipofuscin accumulation quantified after fission inhibition with Mdivi-1 during PQ-induced SIPS in Lon protease (Lon) deficient HeLa cells. Statistically significant differences to the corresponding control (untreated) are indicated by ‘*’, comparison to the corresponding SIPS control are marked by ‘#’.