Table 1. Mitochondrial membrane potential and cell survival.
| Cells | Stimulated (%) | Primary (%) | Secondary (%) | Tertiary (%) |
|---|---|---|---|---|
|
Loss of ψm | ||||
| Dead | 100.0 | 85.7 | 61.1 | 76.9 |
| Alive | 0.0 | 14.3 | 38.9 | 23.1 |
|
Retention of ψm | ||||
| Dead | — | 36.3 | 11.0 | 5.8 |
| Alive | — | 73.7 | 88.9 | 94.2 |
Mitochondrial membrane and cell survival or death were analyzed based on the experiments described in Figure 5. Loss and retention of mitochondrial membrane potential (as assessed by changes in TMRM signal) were significantly correlated with cell survival (alive) and cell death (dead). Cell death was based on the complete loss of TMRM fluorescence. Results from three independent experiments were averaged to obtain the percentile numbers. Each comparison (e.g., primary cell, loss of a ψm, dead versus alive percentile) was found to be significant at P <0.001 using a χ2-test.