Figure 3.

S100A1-mediated rescue of contractile dysfunction in vitro. (A) Efficiency of adenovirus-mediated gene transfer in FCs. Representative transmission (left) and GFP emission images (right) from FCs 24 hours after adenoviral infection. Upper left: FCs-AdGFP transmission; upper right: FCs-AdGFP, 510-nm emission; lower left: FCs-AdS100A1 transmission; lower right: FCs-AdS100A1, 510-nm emission. Scale bar: 100 μm. (B) Restoration of S100A1 protein levels in FCs 24 hours after S100A1 gene transfer. Representative results of Western blots for SERCA2, NCX, CSQ, cardiac actin, GFP, PLB, and S100A1 from homogenates both of untreated NFCs and FCs and AdGFP- and AdS100A1-transfected FCs. Note that GFP is only expressed in adenovirus-treated failing cells. Data are shown from 2 different representative preparations (animals 82 and 93). (C) Rescue of contractile function in FCs after adenoviral S100A1 gene transfer. Original tracings of FS (shown as downward deflection) from a representative NFC, FC, FC-AdGFP, and FC-AdS100A1. (D) Normalization of FS (upper panel), rate of cellular shortening (_dl/dt; μm/ms; middle panel), and rate of cellular relengthening (+dl/dt; μm/ms; lower panel) in FCs after S100A1 gene addition (n = 40 cells from 4 different preparations in each group; *P < 0.01 compared with NFCs; **P < 0.01 compared with FCs and FCs-AdGFP); P = NS, NFCs vs. FCs-AdS100A1. Data are presented as mean ± SEM.