Skip to main content
. 2004 Dec 1;114(11):1676–1685. doi: 10.1172/JCI22218

Figure 2.

Figure 2

Effect of radiation and PGE2 analogs on AKT phosphorylation. HCT-116 cells were treated with DMSO, 10 μM dmPGE2, or one of the EP receptor agonists, butaprost (10 μM), PGE1 alcohol (1 μM), or 17-phenyltrinor-PGE2 (17-PT-PGE2; 10 μM), irradiated at 6 Gy, and lysed at the indicated times. Duplicate lysates were combined, proteins were separated by SDS-PAGE, and phosphorylated AKT (P-AKT) was detected using a phosphospecific antibody. Blots were stripped and reprobed for AKT. Upper panel: Densitometry of Western blot, showing phospho-AKT (P-AKT) corrected to total AKT. Squares, DMSO; circles, dmPGE2; triangles, butaprost; diamonds, PGE1 alcohol; X’s, 17-phenyltrinor-PGE2. Lower panel: Representative Western blot. Data are representative of at least 3 separate experiments.