Figure 7.

NK-mediated capturing of soluble immune complexes. (A) ANK cells (1 × 10⁶) were incubated with recombinant HA or BSA proteins (0.5 μg/ml) and pooled anti-HA or control sera (1 μl per well), for 180 minutes at 37_C. The cells were subsequently stained for surface expression of CD16. MFI levels are indicated for CD16^– (upper left corner) and CD16⁺ NK cells (upper right corner). (B) The proliferative response of 5 × 10⁴ OFR3 clone T cells to different concentrations of HA or BSA proteins, in the presence of a fixed concentration of pooled anti-HA or control sera (1 μl per well). Irradiated ANK cells (1 × 10⁵) were used as APCs. The cells were cultured for 48 hours and pulsed with [³H]thymidine for the last 24 hours. Values are mean ± SD for triplicate samples. Prior to harvesting, 100 μl of supernatant was taken up for ELISA measurement of IL-2 and IFN-γ. Data are representative of 3 separate experiments.