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. 2017 Feb 6;3:16064. doi: 10.1038/cddiscovery.2016.64

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Copyright © 2017 The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Schematic representation of the Master Bank cells. iPS-derived MSCs were expanded from the P0 Feeder Bank to generate cells for the P6 Working Bank. Cells from the P6 Working Bank were expanded to P7 cells and then P7 cells were transfected with a lentivirus designed to express genes for CD gene and UPRT (CD::UPRT). As indicated in the text, cells from the P0 Feeder Bank could be expanded about 10⁶-fold to generate cells for the P6 Working Bank as needed.