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. Author manuscript; available in PMC: 2017 Feb 6.
Published in final edited form as: Cold Spring Harb Protoc. 2016 Jan 4;2016(1):pdb.prot086496. doi: 10.1101/pdb.prot086496

Figure 2.

Figure 2

GFP::LGG-1 expression in hypodermal seam cells of daf-2(e1370) mutants A. daf-2(e1370) mutants grown on OP50 E. coli, at 15°C, display a diffuse localization of GFP::LGG-1. B. daf-2(e1370) mutants grown on OP50 E. coli, at 25°C, display an increase in GFP::LGG-1 positive puncta (up to 12 GFP::LGG-1 positive puncta/seam cell) that represent early autophagic structures or autophagosomes. C. daf-2(e1370) mutants grown on control RNAi E. coli (transformed with empty vector, L4440), at 25°C, display the characteristic GFP::LGG-1 positive punctate structures. D. daf-2(e1370) mutants fed bec-1 RNAi, and raised at 25°C, display an increase in GFP::LGG-1 expression and large GFP::LGG-1 positive aggregates