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. 2017 Feb 6;7:41916. doi: 10.1038/srep41916

Figure 4. Inhibitory effect of resveratrol on TGFβ/Smad3-stimulated SMC de-differentiation in vitro.

Figure 4

Rat aortic SMCs were infected with AdGFP (control) or AdSmad3 followed by pre-incubation with vehicle (DMSO) or resveratrol (50 μM) or rapamycin (200 nM) for 2 h, and then treated with solvent (for AdGFP control) or TGFβ1 (5 ng/mL) for 24 h. Cells were then used for Western blotting of calponin (A) or SM-MHC (B), or staining with Calcein for cell area measurement (C). Quantification: mean ± SEM of three independent experiments; normalization to β-actin; *P < 0.05 compared to AdGFP control; #P < 0.05 compared to AdSmad3 + TGFβ1 without pre-incubation with a drug.