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. 2017 Feb 6;7:41916. doi: 10.1038/srep41916

Figure 6. Inhibitory effect of resveratrol on TGFβ/Smad3-stimulated mTOR activation in vitro.

Figure 6

Rat aortic SMCs were infected with AdGFP (control) or AdSmad3 followed by pre-incubation with vehicle (DMSO), resveratrol (50 μM), rapamycin (200 nM), or LY249004 (20 μM) for 2 h, and then treated with solvent or TGFβ1 (5 ng/mL) for 24 h. Cells were then collected for Western blotting analysis of KLF5 (A), p-mTOR (B) or p-S6K (C). Quantification: mean ± SEM of three independent experiments; normalization to β-actin or to total protein; *P < 0.05 compared to any of the first 3 conditions; #P < 0.05 compared to AdSmad3 + TGFβ1 without pre-incubation with a drug.