Figure 2. Impact of viral infection on microglial cell activating FcγR expression.

BMNCs were extracted from brains of uninfected and MCMV-infected mice at 0, 5, 30, 60 and 90 dpi. Microglia were first identified as CD45^intCD11b^hi cells and subsequently stained for activating FcγRs (FcγRI, FcγRIII and FcγRIV). Flow cytometric contour plots are representative of two separate experiments using 4–6 mice per time point. Gating control is the Fcer1g strain of mice that lacks activating FcγRs. Data presented show mean frequency of microglial cells expressing each FcγR at the corresponding time point.