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. 2017 Feb 6;7:41889. doi: 10.1038/srep41889

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Copyright © 2017, The Author(s)

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Primary murine microglial cells were either unstimulated (C) or stimulated with IL-4 or IFNγ for 6 h and 24 h. The cDNA synthesized from 1 μg RNA was amplified and fold change of mRNA expression of FcγRs (A) FcγRIV; (B) FcγRIIB; (C) FcγRI and (D), FcγRIII relative to unstimulated control was quantified using the 2^−∆∆Ct method and normalized to the housekeeping gene HPRT. Data shown are representative of two experiments.