Figure 6. Chromatin condensation and DNA break studies were carried out to assess the condition of the nuclear set up of the control and NT treated cells.

Prominent chromatin condensation and plasma membrane permeability (c,d) was recorded with the NTs treated group. (Scale bars represent 10 μm). The NF-κB expression was elevated in the treatment group when compared to control (e). Comet analysis confirmed significant increase in number of comet tails in treatment group (f). Consistent DNA breaks could also be observed with the H2AX test in the NTs group (k) when compared to the controls (h). (Scale bars represent 500 μm). This nuclear condensation and DNA alteration study is correlative of the ROS induction previously observed.