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. 2017 Feb 6;7:41840. doi: 10.1038/srep41840

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Copyright © 2017, The Author(s)

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(A) Protocol for gene expression analyses of ESCs, Flk1⁺ MPCs, PDGFRα^-Flk1⁻ cells, PCBs, and M⁺CMs. (B and C) Relative mRNA expression levels of pluripotency- (oct4, nanog and sox2), mesoderm- (brachyury, mesp1), cardiac transcription factor- (meis1, tbx5, nkx2.5, gata4, isl1 and hand2), cardiac sarcomere protein- (tnnt2 and myl7), and mitochondrial biogenesis- (pgc1α) related genes in the indicated cells. All values are relative to that of PDGFRα⁻Flk1⁻ cells. In all graphs, *p < 0.05 and **p < 0.01 versus indicated cells.