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. 2017 Feb 6;7:41823. doi: 10.1038/srep41823

Figure 5. Inhibition of canonical Wnt signaling does not diminish Wnt7a’s inhibition of IL-1β activity in human chondrocytes.

Figure 5

(a) RT-PCR analysis of Axin2 mRNA expression in nHACs after infection with lenti-GFP or lenti-Wnt7a-GFP, and cultured with or without IL-1β (5 ng/mL). (b) Western blot analysis of nuclear β-catenin in nHACs after infection with lenti-GFP or lenti-Wnt7a-GFP in the presence or absence of IL-1β (5 ng/mL). TATA-binding protein (TBP) served as a loading control. Original films for cropped images can be found in the supplementary information file. (c) RT-PCR analysis of Axin2, MMP1, MMP13 and iNOS gene expression after treatment with DKK-1 (250 ng/mL), on nHACs infected with lenti-GFP or lenti-Wnt7a-GFP, and cultured with or without IL-1β (5 ng/mL). Each experiment had three biological repeats/treatment, and at least three independent experiments were performed. A student’s t-test was used for evaluating the statistical significance between the gene expression of lenti-GFP and lenti-Wnt7a cells under each individual experimental condition. All data are shown as mean ± SEM. *p < 0.05.