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. 2017 Feb 6;7:41823. doi: 10.1038/srep41823

Figure 6. Inhibition of Akt signaling does not diminish Wnt7a’s inhibition of IL-1β activity in human chondrocytes.

Figure 6

(a) Western blot analysis of Akt protein expression after nHACs were infected with lenti-GFP or lenti-Wnt7a-GFP and cultured with or without IL-1β (5 ng/mL). Results demonstrated that phosphorylated Akt was upregulated with Wnt7a ectopic expression compared to lenti-GFP controls. Original films for cropped images can be found in the supplementary information file. (b) RT-PCR analysis of MMP1, MMP13 and iNOS gene expression after treatment with Akt inhibitor AZD5363 (1 or 10 uM) on nHACs infected with lenti-GFP or lenti-Wnt7a-GFP and cultured in the presence of absence of IL-1β (5 ng/mL). Each experiment had three biological repeats/treatment, and at least three independent experiments were performed. Analysis of variance (ANOVA) with post-hoc tests was used for evaluating the statistical significance between the gene expression of lenti-GFP and lenti-Wnt7a cells across all of experimental conditions. All data are shown as mean ± SEM. *p < 0.05.