Figure 5.

SMGRKO mice develop left ventricular cardiac fibrosis. Representative images of picrosirius red/fast green-stained sections of formalin-fixed paraffin-embedded hearts from SMGRKO mice and littermate controls (A, B and C). Assessment of picrosirius red staining of collagen in LV of SMGRKO mice (black/hatched/striped bars) compared with controls (white bars). Four fields of view were scored from the LV of each mouse by an investigator blind to genotype (scoring system described in ‘Materials and methods’ section). (A) 12-week-old males and females (N = 7–10). (B) 6-week-old males (N = 10). (C) 2-day-old males and females (N = 8–10). Data are means ± s.e.m. and were analysed using unpaired t-tests. *P < 0.05, ***P < 0.001.