Fig. 4.

Role of IL-33 in cytokine production by mT_H2 cells. KJ-1-26⁺ T_H2EPs from WT or Bcl6-KO mice were adaptively transferred into naïve WT BALB/c mice. Twenty-eight days later, mT_H2 cells were isolated from the recipient spleens. These mT_H2 cells were incubated ex vivo with IL-33 or medium alone for 3 h before reactivation with anti-CD3 Abs. (A) FACS analysis for IL-4– and IL-13–producing cells among T_H2 cells (gate: ST2⁺ populations) after restimulation. The figure shows representative FACS data. The numbers in each corner represent the percentages of T cells in the column. (B) Percentage of the indicated cytokine-positive population (gate: ST2⁺ T_H2 cells). (C) Protein concentration in culture supernatants at 48 h and (D) mRNA levels at 24 h for IL-4, IL-5, and IL-13 produced by the sorted ST2⁺ T_H2 cells after restimulation. (B–D) Data are shown as means ± SEMs (n = 7–8). All results are representative of eight independent experiments with similar outcomes. NS, not significant. *P < 0.05; **P < 0.01.