Fig. 6.
Role of Bcl6 on the effects of IL-33 regarding mTH2 cell-mediated allergic responses. WT and Bcl6-KO KJ-1+ TH2EPs (3 × 107) were adoptively transferred into IL-33–WT or –KO BALB/c background recipient mice. (A) The recipient mice were given two intratracheal administrations of OVA or LPS plus OVA at a 12-h interval on day 28 posttransfer. Levels of Il4, Il5, and Il13 mRNA in mTH2 cells isolated from MLNs 36 h after the final challenge (day 30) are shown. (B) Recipients were challenged with three instillations of either OVA or saline at 5-d intervals beginning on day 1 or 28 posttransfer. Serum from each recipient was subjected to analysis to determine the titer of OVA-specific IgE Abs at 48 h (day 40) after the final challenge. Data are shown as means ± SEMs with (A) n = 7–10 or (B) n = 10–13. (A) Results are representative of three independent experiments with similar outcomes. (B) Data are pooled from five independent experiments. NS, not significant. *P < 0.05; **P < 0.01.