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. 2017 Jan 17;114(5):E879–E886. doi: 10.1073/pnas.1620315114

Fig. 6.

Fig. 6.

WNK4-RRXS phosphorylation promotes WNK4 T-loop autophosphorylation. (A) WNK4 Ser-322 phosphorylation was assessed in immunoprecipitated WNK4 from HEK293 cells transfected with WNK4-WT, WNK4-3A (S64A, S1169A, S1196A) or WNK4-5A (S47A, S64A, S1169A, S1180A, S1196A) and stimulated with vehicle or forskolin for 30 min before lysis. (B) Results of quantitation. White bars, nonstimulated cells; Black bars, forskolin-stimulated cells. (C) Same as in A, but WNK4 Ser-322 phosphorylation was assessed in immunoprecipitated WNK4 from lysates of cells stimulated with vehicle or AngII for 30 min before lysis. The PKC inhibitor BIM was also added in the indicated groups. (D) Results of quantitation. White bars, nonstimulated cells; Black bars, AngII-stimulated cells. Data are means ± SEM; *P < 0.01 vs. WT-nonstimulated (n = 3–6 in each group). See also Fig. S4 C and D.