Fig. S3.

BrdU administration does not affect parasite replication as determined by cluster analysis. The percent of PIP-infected cells containing the indicated number of parasites was determined from mice subjected or not to BrdU dosing (6 systemic, 6 in situ injections of BrdU over 18 h, as described in Materials and Methods). Data from control mice are derived from analysis of images collected for immunolocalization of PIP-containing host cells. Data from BrdU-treated mice were obtained by analysis of images from tissue stained to detect parasite histones and iNOS. The graph shows the mean ± SEM for each cluster/treatment; n.s., not significant (P = 0.662) by two-tailed ANOVA with Bonferonni post hoc analysis. n = 3 mice per group; 130 infected cells (BrdU-treated) or 223 infected cells (control).