Surface marker expression and morphology is comparable between GM-CSF/IL-3 and IL-3-elicited basophils. (A) Cell surface expression of FcεRIα, IL-3Rα, Thy1, and 2B4 on IL-3- and GM-CSF/IL-3-elicited BMBAs is shown. Relative expression of each molecule is shown (mean ± SEM; n = 3 each). (B) May-Grünwald-Giemsa staining of IL-3- and GM-CSF/IL-3-elicited BMBAs. (Scale bar, 10 μm.) Data are representative of at least three independent experiments. n.s., no significant differences.