Fig. 1.
Analysis of TKL and F-TKL formation with constructs of DEBS Mod2, Mod3TE, and Mod6TE. (A) Reaction scheme for the TKL formation assay with Mod3TE. KR*, natively inactive ketoreductase domain. (B) Endpoint and initial rate quantification of TKL and F-TKL formation with 10 µM DEBS Mod3TE constructs and KSAT3. (C) Endpoint quantification of TKL and F-TKL formation with 10 µM DEBS Mod2 and Mod6TE constructs and KSAT6. Only KS, AT, and ACP domains are shown in the labels for Fig. 1 B and C. All data are mean ± SE (n = 3). n.d., not detected; –, data not collected.