Table S2.
Oligonucleotides used in this study
| Designation | Gene | Sequence (5′–3′) |
| pET28b(+) | PxaTPS5-forward | TATCATATGGCTGGCGTTTCTGTTG |
| PxaTPS5-reverse | TATGTCGACTTAAAGTGGGAGCGGATCA | |
| PxaTPS8-forward | TATCATATGATACGGTCAAATACTTCG | |
| PxaTPS8-reverse | TGAGAGCTCTCATAAAGACACAGCTTG | |
| pET28b(+) PxaTPS8 variants | R558A-sense | CCACGTTTCTATGTGCAGTGAACCTC |
| R558A-antisense | GAGGTTCACTGCACATAGAAACGTGG | |
| S696I-sense | ATGTAAACCAATCGTGACCTTGCTGTTC | |
| S696I-antisense | GAACAGCAAGGTCACGATTGGTTTACAT | |
| S696V-sense | CTATATGTAAACCAACCGTGACCTTGCTGT | |
| S696V-antisense | ACAGCAAGGTCACGGTTGGTTTACATATAG | |
| G697S-sense | ATCGCTATATGTAAACTACTCGTGACCTTGCTG | |
| G697S-antisense | CAGCAAGGTCACGAGTAGTTTACATATAGCGAT | |
| Y564T-sense | CCATAGTGTAAGTTTCCACGTTTC | |
| Y564T-antisense | GAAACGTGGAAACTTACACTATGG | |
| Y564V-sense | CCATAGTGTAAACTTCCACGTTTC | |
| Y564V-antisense | GAAACGTGGAAGTTTACACTATGG | |
| Y564H-sense | ATAGAAACGTGGAACATTACACTATGGC | |
| Y564H-antisense | GCCATAGTGTAATGTTCCACGTTTCTAT | |
| Y564F-sense | CCATAGTGTAAAATTCCACGTTTC | |
| Y564F-antisense | GAAACGTGGAATTTTACACTATGG | |
| Y564W-sense | TAGAAACGTGGAATGGTACACTATGGCAT | |
| Y564W-antisense | ATGCCATAGTGTACCATTCCACGTTTCTA | |
| A591T-sense | AATTGTGTGGAATCACTACTTGTATCGATG | |
| A591T-antisense | CATCGATACAAGTAGTGATTCCACACAATT | |
| A591Q-sense | ATTGTGTGGAATCCCAACTTGTATCGAT | |
| A591Q-antisense | ATCGATACAAGTTGGGATTCCACACAAT | |
| H670A-sense | TATAATTGATGCGGCTCTGGTAGAAGC | |
| H670A-antisense | GCTTCTACCAGAGCCGCATCAATTATA | |
| H670Y-sense | TATAATTGATGCGTATCTGGTAGAAGCG | |
| H670Y-antisense | CGCTTCTACCAGATACGCATCAATTATA | |
| H670W-sense | TATAATTGATGCGTGGCTGGTAGAAG | |
| H670W-antisense | CTTCTACCAGCCACGCATCAATTATA | |
| A701C-sense | GTGAGGATGGGCAATATACATATATGTAAACCAC | |
| A701C-antisense | GTGGTTTACATATATGTATATTGCCCATCCTCAC | |
| A701L-sense | GATGGGCAATATCAGTATATGTAAACCACTCG | |
| A701L-antisense | CGAGTGGTTTACATATACTGATATTGCCCATC | |
| G735S-sense | GCCTCCACCATTAGTCGACTAAGTGGT | |
| G735S-antisense | ACCACTTAGTCGACTAATGGTGGAGGC | |
| G735W-sense | ACTTGGCCTCCACCATTTGGCGACTAAGT | |
| G735W-antisense | ACTTAGTCGCCAAATGGTGGAGGCCAAGT | |
| G739N-sense | TTGGTCGACTAAGTAATGACATGAACG | |
| G739N-antisense | CGTTCATGTCATTACTTAGTCGACCAA | |
| G739D-sense | GGTCGACTAAGTGATGACATGAACGCC | |
| G739D-antisense | GGCGTTCATGTCATCACTTAGTCGACC | |
| A743T-sense | GGTGACATGAACACCTACAAGATTGACC | |
| A743T-antisense | GGTCAATCTTGTAGGTGTTCATGTCACC | |
| PxaTPS8:helix H chimera 5′-forward | CAAATGGGTCGCGGATCCATGTCAAATACTTCGAAATATG | |
| PxaTPS8:helix H chimera 3′-reverse | GGTGGTGGTGCTCGAGTTATAAAGACACAGCTTGAACG | |
| PxaTPS8:helix H chimera internal-forward | GGTCTTTGTGTCGTCTGCCAGGCGGGAAATGAGGCTGTTAAGCTCTGTGAGAACACGCCTAGACAGAGTATCAATTTCCTGCA | |
| PxaTPS8:helix H chimera internal-reverse | GCCTCATTTCCCGCCTGGCAGACGACACAAAGACCTACAAAGCTGAGAAGGCTCGTGGAGAAGAATCTTCATGCATATCGTGTTATATG | |
| pLIFE33 | PxaTPS5-forward | GGCTTAAUAATGGCTGGCGTTTCTGTTG |
| PxaTPS5-reverse | GGTTTAAUTTAAAGTGGGAGCGGATCAATTAGAC | |
| PxaTPS8-forward | GGCTTAAUAATGTCAAGATTTACATCTGCTGC | |
| PxaTPS8-reverse | GGTTTAAUTTATAAAGACACAGCTTGAACGAG | |
| pESC-His | PxaTPS8-forward | GAATTCAACCCTCACTAAAGGGCGGCCGCATGACGAAAAGGAGTGAAGCAGATG |
| PxaTPS8-reverse | CTTGTAATCCATCGATACTAGTGCGGCCGCTTATAAAGACACAGCTTGAACGAGAGTTC | |
| qRT-PCR | PxaTPS5-forward | CTTGATTCGAAGAACGGCCAA |
| PxaTPS5-reverse | TCGTTGATTCTCCATCTCCTGC | |
| PxaTPS8-forward | TGCTAGAAACGCTTGGGAGG | |
| PxaTPS8-reverse | ATGGTGGAGGCCAAGTGATG | |
| EF-1α-forward | CCTGGCCATCGTGACTTCAT | |
| EF-1α-reverse | AAGTGAACGCCAATAGGGCA |