Fig. 5.

Identification of amino acid residues critical for HEV ORF3’s ion channel activity. (A) HepG2C3A cells lentivirally transduced with HA-tagged wild type or the indicated ORF3 mutant were stained with anti-HA and anti-calnexin antibodies. Nuclei were stained with Hoechst dye. Shown are representative images of triplicate experiments. (B) Current–voltage relationship of X. laevis oocytes expressing HEV wild type or the indicated ORF3 mutants. Experiments were conducted as detailed in Fig. 3. Data represent the mean + SD (n = 5).