Fig. S1.

(A) Schematic representation of the wild-type HEV and rHEVΔORF2/3[Gluc] genomes and the bicistronic lentiviral constructs for expressing ORF2 and ORF3. (B) Cell culture supernatants from naïve HepG2C3A, or HepG2C3A cells transduced with HEV ORF2 and ORF3, M2, or M2(A30P), were collected 5 d posttransfection with rHEVΔORF2/3[Gluc] RNA. Naïve HepG2C3A cells were incubated with these supernatants. After 12 h, cells were washed and intracellular total RNA was extracted 4 d later for RT-qPCR assay to quantify HEV RNA. HEV RNA was normalized to the housekeeping gene GAPDH. Data represent the mean ± SD (n = 3). *P < 0.05, **P < 0.01, and ***P < 0.001. LOD, limit of detection.