Fig. S6.

Ion channel activity of different ORF3 mutants. (A) Wild type or alanine mutants of HEV ORF3 expressed in X. laevis oocytes localize to the plasma membrane. Water-injected and HEV ORF3 mRNA-injected oocytes were immunolabeled with polyclonal ORF3 antibody and analyzed by confocal microscopy. (B) Current–voltage relationship of X. laevis oocytes expressing HEV wild type or the indicated ORF3 mutants. Experiments were conducted as detailed in Fig. 3. Data represent the mean + SD (n = 5). (C) Summary of the features of triple alanine ORF3 mutants that do not support HEV release.