Fig. S5.

Effects of filter mutations on HsTPC2 selectivity. (A) Representative I-V curves of HsTPC2 filter mutants recorded on inside-out patches, with 150 mM Na⁺ in the pipette and 150 K⁺ in the bath solution. (B) I-V curve of the HsTPC2 triple mutant (A272S/V652M/N653G). The current was recorded with 100 mM Ca²⁺ in the pipette and 150 Na⁺ in the bath solution. All statistics of reversal potentials and calculated permeability ratios are summarized in Table 1.