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Effects of Plk1 and Cdk on the disassembly of MCC and its subcomplexes. The release of Cdc20 from anti–BubR1-bound recombinant mitotic checkpoint complexes was determined as described in Materials and Methods. Where indicated, additions were at the following concentrations: ATP, 2 mM; Cdk1–cyclin B, 1,000 units; Plk1-WT and Plk1-N172A, 80 nM.
