Table S1.
Amount of precursor 17S rRNA in various ribosomal fractions of the strains analyzed
Strain name | Description* | Sucrose gradient fraction | ||||
No. 5, pre-30S | No. 6, pre-30S | No. 7, 30S | No. 8, 30S | Nos. 13/14, 70S | ||
MRG03 | WT | 58.0 ± 5.9 (n = 4) | 42.3 ± 2.9 (n = 5) | 35.6 ± 3.6 (n = 5) | 29.9 ± 3.9 (n = 5) | 7.6 ± 2.4 (n = 5) |
MRG07 | ∆rsgA | 75.3 ± 4.1 (n = 3) | 75.1 ± 3.6 (n = 3) | 71.7 ± 3.6 (n = 3) | 75.5 ± 5.2 (n = 3) | 37.2 ± 2.7 (n = 3) |
MRG08 | ∆rsgA (pRGSA) | 67.2 ± 4.9 (n = 3) | 43.1 ± 8.5 (n = 3) | 28.9 ± 8.5 (n = 3) | 28.9 ± 5.7 (n = 3) | 9.3 ± 0.5 (n = 3) |
MRG04 | ∆lepA | 69.8 ± 2.3 (n = 5) | 54.4 ± 2.1 (n = 5) | 49.1 ± 4.3 (n = 5) | 37.2 ± 5.9 (n = 5) | 9.7 ± 3.6 (n = 5) |
MRG05 | ∆lepA (pLEPA) | 65.9 ± 4.8 (n = 6) | 40.5 ± 3.4 (n = 6) | 35.2 ± 4.4 (n = 6) | 21.0 ± 4.3 (n = 6) | 7.2 ± 1.1 (n = 6) |
MRG102 | ∆lepA (pRB34) | 73.9 ± 1.3 (n = 3) | 56.1 ± 0.7 (n = 3) | 43.8 ± 2.6 (n = 3) | 35.7 ± 2.8 (n = 3) | 10.1 ± 0.7 (n = 3) |
Data correspond to percent of total SSU rRNA (mean ± SEM), with the number of independent replicas (n) indicated.
All strains are isogenic and carry either the plasmid indicated or the empty vector.