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. 2017 Jan 17;114(5):E887–E896. doi: 10.1073/pnas.1614380114

Table S1.

Primers used in this study

Name Purpose Primer sequence (5′–3′)
PIN1 S2A Mutagenesis of S252 (S2) to A252 (S2A) in PIN1:YFP phos-CCACGTGGCGCTAGTTTTAATCATAC
PIN1 S3A Mutagenesis of S290 (S3) to A290 (S3A) in PIN1:YFP phos-ACTCCGAGACCTGCCAACTACG
PIN1 S1E Mutagenesis of S231 (S1) to E231 (S1E) in p002:PIN1 phos-ACACCTAGACCTGAAAATCTAAC
PIN1 S2E Mutagenesis of S252 (S2) to E252 (S2E) in p002:PIN1 phos-GCCACGTGGCGAAAGTTTTAATCATAC
PIN1 S3E Mutagenesis of S290 (S3) to E290 (S3E) in p002:PIN1 phos-ACTCCGAGACCTGAAAACTACG
PIN1 S4E Mutagenesis of S271 (S4) to E271 (S4E) in p002:PIN1 phos-TGGTCGGAACGAAAACTTTGGTC
GW-PID-FW Amplification and cloning of PID CDS in pGW-35S-MYC and pExtag-YFP-GW GGGGACAAGTTTGTACAAAAAAGCAGGCTTCATGTTACGAGAATCAGACGGT
GW-PID-RV1 Amplification and cloning of PID CDS in pGW-35S-MYC and pExtag-YFP-GW GGGGACCACTTTGTACAAGAAAGCTGGGTCTCAAAAGTAATCGAACGCCGCTG
pin1 LP pin1 (SALK_047613) mutant genotyping: pin1 LP CAAAAACACCCCCAAAATTTCTC
pin1 RP pin1 (SALK_047613) mutant genotyping: pin1 RP AATCATCACAGCCACTGATCC
LBb1.3 pin1 (SALK_047613) mutant genotyping: LBb1.3 ATTTTGCCGATTTCGGAAC
AGC1-5 FW Amplification and cloning of AGC1-5 GGGGACAAGTTTGTACAAAAAAAGCAGGTTAATGGACTTAGCTTCTAAGAAGAACAC
AGC1-5 RV Amplification and cloning of AGC1-5 GGGGACCACTTTGTACAAGAAAGCTGGGTACTAAAAGTACTCGAAATCTATATACGCC
KIPK FW Amplification and cloning of KIPK GGGGACAAGTTTGTACAAAAAAGCAGGCTTAATGGGGTCGTTTGCAGG
KIPK RV Amplification and cloning of KIPK GGGGACCACTTTGTACAAGAAAGCTGGGTATTAAAACAACTCGAACTCAAGATGATC
D6PKL3 Amplification and cloning of D6PKL3 GGGGACCACTTTGTACAAGAAAGCTGGGTCCTGATAGTGAAAGAAATCACAAATCAG
D6PKL3 Amplification and cloning of D6PKL3 GGGGACAAGTTTGTACAAAAAAGCAGGCTTAATGGATTCTTCTTCATCAGT

phos, phosphorylation.