Fig. 2.

The host’s transcriptional response to Y. pseudotuberculosis. (A) Volcano plot obtained from DESeq2 analysis of uninfected and infected Peyer’s patches RNA pools. (B) Heat map of the top 50 enriched and depleted host transcripts. Color-coding is based on rlog-transformed read count values. (C) Infection-mediated activation of extrinsic tissue factor-dependent coagulation cascade genes. On IP32953 colonization, extrinsic coagulation cascade transcripts are highly enriched in infected Peyer’s patches. Fibrin clotting is typically induced by bacterial antigens, such as LPS, to trap the invading bacteria and prevent further dissemination. In addition, stimulation of the extrinsic coagulation pathway is triggered by the neutrophil-specific collagenase MMP8, which activates the tissue factor via cleavage of TFPI.