Fig. S2.

Lack of mitochondrial activation of CTLs in the therapy of unresponsive tumor cells (LLC). (A) LLC-bearing mice were treated with PD-L1 mAb with the same schedule as in Fig. 1A. (B) OCRs of DLN CD8⁺ T cells isolated from treated or untreated mice were measured by Seahorse XF^e96 analyzer. Cells were mixed from three mice 2 d after the second therapy. (C) ATP turnover defined as (last rate measurement before oligomycin) − (minimum rate measurement after oligomycin injection) was calculated. Data represent the means ± SEM of six wells. N.S., no significant difference, two-tailed Student t test.