Fig. S7.

T-bet and IFN-γ production was up-regulated in a combination of FCCP with PD-L1 mAb. (A) T-bet and Eomes expression was analyzed by flow cytometry in DLN CD8⁺ T cells from mice treated with PD-L1 mAb and FCCP. Representative FACS data are shown (Upper). The frequency and numbers of T-bet⁺ or Eomes⁺ T cells were calculated in DLN CD8⁺ T cells from mice treated with PD-L1 mAb and FCCP. *P < 0.05, one-way ANOVA. (B) Digested tumor tissues were incubated at 37 °C for 6 h, and IFN-γ was intracellularly stained in the CD8⁺ T cells from mice treated with FCCP. Representative FACS data (Left) and the frequency of IFN-γ⁺ T cells among CD8⁺ T cells are shown (Right). Data represent the means ± SEM of five mice. *P < 0.05, one-way ANOVA. Data are representative of two independent experiments.