Fig. 4.

Water soaking can introduce bacterial cells into the apoplast. N. benthamiana was syringe-infiltrated with either X. gardneri (Xg) or XgΔhrcV at OD₆₀₀ = 0.1. Water soaking was induced at 48 hpi with a 30-μL drop of Tet-resistant X. gardneri (X. gardneri Tet^R, 10⁵ CFU/mL in 1 0 mM MgCl₂) on a wound (arrow). Leaf discs were collected away from the wound site after 5 min (dashed circle), ground in 10 mM MgCl₂, and dilutions were plated on either Rif or Rif + Tet to select for the growth of all X. gardneri or the X. gardneri Tet^R from the water-soaking inoculum, respectively.