Table S3.

PfRH5ΔNL_HS1 structure features compared with PfRH5ΔNL

Structural feature	Contributing mutations*
Mutations at buried positions	D183E, L314F, S286A
Mutations improving helical propensity	I157L, D183E, S370A, T384K, N398E, N464K, S467A
Surface mutations increasing polarity	A233K, M330N, S381A, T384K, L392K, T395N, N398E, N464K
Mutations in homogenous positive patch	K312N, K316N

^*Numbering according to PDB ID code 4WAT.