Fig. 1.

Loss of SREBP2 from glia inhibits neurite outgrowth. SREBP2 was knocked down in C6 rat glioma cells by stable transfection with a lentiviral delivered shRNA against Srebf2 or a control shScramble. (A) Cholesterol content of cells and cholesterol secreted into serum-free media were measured using the Amplex red assay. n = 3 per group. (B and C) The GT1-7 hypothalamic mouse cell line was grown alone or in coculture using transwells with C6 shScramble or C6 shSrebf2, and neurite length was measured using ImageJ (n = 146, 244 and 246 neurites measured, respectively). Student’s t test ***P < 0.001 compared with C6 shScramble. White arrows indicate examples of neurites. (Magnification: 10×.)