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. 2017 Jan 17;114(5):1189–1194. doi: 10.1073/pnas.1620506114

Fig. S2.

Fig. S2.

G-SB2KO mice show little change in brain cell types, expression of Gfap, liver Srebf2, brain cholesterol content, or synaptic proteins. (A) cDNA from whole brains of control and G-SB2KO mice were assessed for relative expression of cell-type–specific markers, Olig2 (oligodendrocytes), Aqp4 (astrocytes), Map2 (neurons), and F4/80 (microglia). n = 5 per group. (B) Expression of Gfap was determined by qPCR in isolated brain regions: cortex, amygdala (Amyg), hypothalamus (Hypo), and hippocampus (Hippo). n = 6 control, 5 G-SB2KO. (C) The presynaptic marker Syntaxin1a and the postsynaptic marker PSD95 were measured by Western blot of proteins from isolated synaptosomes from the cortex and amygdala. n = 3 animals per group, performed in triplicate. (D) Expression of Srebf2 was measured by qPCR in livers from 3-mo-old male mice. n = 3 per group. (E) Mice were perfused with saline then brains were dissected for the brain regions indicated on the graph. Lipids were extracted from the tissue before measurement. n = 6 animals per group. Student’s t test *P < 0.05, **P < 0.01 compared with control.