Fig. 6.

Human excision nuclease assay. (A) Schematic representation of human excision nuclease assay. (B) Excision of 1,2-d(GpG) cisplatin cross-links by excinuclease activity in testicular cancer cell extracts. The substrate (146 bp, Pt-DNA probe) was incubated with CFEs from NTera2 and NTera2 HMGB4^−/− cells. The reaction was run for 75 min and the excision products were resolved on 10% (wt/vol) polyacrylamide denaturing gels (TE buffer, 55–46 mA for 5 h). The position of the main excision product, a 25–30 nt is indicated by a bracket. Replicates of this experiment with two independently prepared cell-free extract sets are reported in SI Appendix, Fig. S8. (C) Percent excision products from the NER assay using CFEs from NTera2 and NTera2 HMGB4^−/− cells in B. Error bar represents SD (n = 3).