Fig. 4.
Three-dimensional reconstruction of mitochondrial nanotunnels in RyR2A4860G+/−. (A) Orthoslice view of cardiac muscle with mitochondrial nanotunnels and microtubules segmented and reconstructed using electron tomograms. Each color identifies a specific mitochondrion (Movie S2), whereas microtubules are highlighted in purple. (B and C) Mitochondria and microtubules are shown in the volume of the section (566 millions voxels) in different orientations. Nanotunnels extend in three dimensions through the volume of the myocyte, passing over each other and appearing as small, round mitochondria on the surface of the section (Movie S3). Microtubules follow their profiles. (D–F) Three representative orientations emphasize the complexity (Movie S4).