Fig. 1. : in silico inferred probe specificity for in situ hybridisation of Paracoccidioides brasiliensis and P. lutzii. Aligned sequences of the internal transcribed spacer 1 (ITS1) rRNA region of Paracoccidioides spp., Histoplasma capsulatum and Emmonsia sp. used for the in silico tests of probe designing for in situ hybridisation of P. brasiliensis and P. lutzii, with fluorophores HRP + Fluorescein and Texas-Red, respectively. The design of specific probes was based on the divergences between the Paracoccidioides species and between the Paracoccidioides genus and other Ajellomycetacea species (H. capsulatum and Emmonsia spp), and other pathogenic yeasts like Cryptococcus sp. (Tremellaceae) and Candida spp. (Saccharomycetaceae).
