Table 2.

LC-MS gradients used for hydrogen exchange screening of the peptide library as shown in Figure 2. The shallow portion of the gradient is denoted by numbers in bold. The B mobile phase was 90% acetonitrile/10% water/01.% formic acid by volume. The A mobile phase (not listed) was 0.1% formic acid.

Early Gradient
Time (min)	0	1	11	17	19	23
%B	2	2	19	95	95	2
Middle Gradient
Time (min)	0	1	2	5	17	21	23	27
%B	2	2	12	12	24	95	95	2
Late Gradient
Time (min)	0	1	2	6	11	16	18	23
%B	2	2	26	26	40	95	95	2