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. Author manuscript; available in PMC: 2017 Feb 6.
Published in final edited form as: J Am Chem Soc. 2016 Jan 21;138(4):1335–1343. doi: 10.1021/jacs.5b11742

Table 3.

Results from screening the eNOS peptide-spiked E. coli peptide library for binding to calmodulin.

Early gradient Middle Gradient Late Gradient Total
Identified features 2474 2759 1431 6664
Unique (– calmodulin) 174 306 215 695
Unique (+ calmodulin) 186 125 95 406
Identified hits1 28 43 46 117
Confirmed hits2 0 1 0 1
1

Hits were identified on the basis of altered hydrogen exchange using the criteria in equations (1) and (2).

2

Hits were confirmed by inspection of the extracted spectra as illustrated in Figures 4 and S2.