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. 2017 Feb 6;40(2):62–70. doi: 10.1097/CJI.0000000000000156

FIGURE 1.

FIGURE 1

Immunization with codon-optimized polynucleotides encoding HPV16-E6/E7 induce acute and long-lasting humoral and cytotoxic immune responses. Mice were immunized intradermally into the ear twice 3 weeks apart with HPV16-E6/E7 DNA vaccine (NTC-HPV16-E6/E7) or empty vector (NTC-empty). A and B, E7-specific IgG antibody responses of NTC-HPV16-E6/E7 immunized mice (grey circles) were determined in serum at week 0 and 5 by ELISA (A). E6-specific and E7-specific cytotoxic T-cell responses were measured by ELISPOT in splenocytes at week 5 after immunization (B) (n=10). C and D, E7-specific IgG antibody responses of NTC-HPV16-E6/E7 immunized mice (grey circles, n=5) or NTC-empty immunized mice (black squares, n=3) were determined in serum at week 0 and 5, 13, and 21 by ELISA (C). E6-specific and E7-specific T-cell responses were measured in splenocytes at week 21 by IFNγ ELISPOT (D). Each data point represents means of technical replicates of 1 individual animal. Mean values of averaged individuals and SDs are indicated. *P≤0.05, ***P≤0.001, ****P≤0.0001.