Skip to main content
. 2017 Feb 6;40(2):62–70. doi: 10.1097/CJI.0000000000000156

FIGURE 4.

FIGURE 4

E7TCR269 mice immunized with HPV16-E6/E7 DNA vaccine reject HPV16-E7-expressing skin grafts. E7TCR269 mice were immunized with HPV16-E6/E7 DNA vaccine or vector expressing an irrelevant protein (HSV gD2) intradermally into the ear twice 3 weeks apart. One week after the second immunization, mice received skin transplants from C57BL/6 and K14E7 animals. Grafts were measured weekly (n=4–6). A, Photographs taken 28 days after grafting. Shown is 1 representative per group. B, Graft size reduction was calculated based on graft size on day 7 after grafting, when bandages were removed. C, E6-specific and E7-specific T-cell responses were measured in splenocytes at the end of the grafting study by IFNγ ELISPOT. Each data point represents means of technical replicates of 1 individual animal. Mean values of averaged individuals and SDs are indicated. D, E7-specific IgG antibody responses were determined in serum at the end of the grafting study by ELISA. Each data point represents means of technical replicates of 1 individual animal. Mean values of averaged individuals and SDs are indicated. *P≤0.05, **P≤0.01.