Table 2. Primer information.

Primer	Forward primer sequence(5’-3’)	Reverse primer sequence(5’-3’)	Amplicon size (bp)	Target region	Primer concentration (μmol/L)
BTV	AGGTGACACTATAGAATAAGGGTAACTCACAGCAAACTCAA	GTACGACTCACTATAGGGAGAGCAGCCTGTCCATCCC	136	VP7	0.2
FMDV	AGGTGACACTATAGAATAGCCGTGGGACCATACAGG	GTACGACTCACTATAGGGAAAGTGATCTGTAGCTTGGAATCTC	166	3D	0.2
IBRV	AGGTGACACTATAGAATAGCGTCATTTACAAGGAGAACATC	GTACGACTCACTATAGGGAATCTCGCCCATGCCCAC	188	gB	0.2
BRV	AGGTGACACTATAGAATACAGTGGCTTCCATTAGAAGCAT	GTACGACTCACTATAGGGAGGTCACATCCTCTCACTA	211	VP6	0.2
VSV	AGGTGACACTATAGAATAAAACTACTGGACGGGCTTGA	GTACGACTCACTATAGGGATGAGATGCCCAAATGTTGC	278	N	0.2
BVDV	AGGTGACACTATAGAATAGTGAGTTCGTTGGATGGC	GTACGACTCACTATAGGGATATGTTTTGTATAAGAGTTCATTTG	308	5’-UTR	2

Universal tag sequences were underlined. Chimeric primers were synthesized using universal primers and gene-specific primers.