Skip to main content
. 2017 Feb 6;12(2):e0171328. doi: 10.1371/journal.pone.0171328

Fig 2. Effect of reducing agents on SRPK1 electrophoretic mobility.

Fig 2

(A) Extracts from K562, MEL, MOLT-4, JM1, 293T and HeLa cells were analyzed under non-reducing conditions (i.e., without DTT) or reducing conditions (in the presence of 90 mM DTT) on 10% SDS-polyacrylamide gels. The proteins were then transferred to nitrocellulose, and SRPK1 was detected with an anti-SRPK1 monoclonal antibody. (B) GST-SRPK1 was analyzed in the absence or in the presence of 90 mM DTT on 10% SDS-polyacrylamide gels and stained with Coomassie Blue.